The Pelegri Lab
Research Photos
Figure 1: In situ hybridization for vasa RNA on four-cell stage embryos. At this stage vasa RNA is normally localized as four aggregates at the periphery of the furrow planes. In aura mutant embryos, vasa RNA does not aggregate at the furr ows, but appears to remain in regions surrounding the forming cells. In nebel mutant embryos, vasa RNA aggregates at the furrows but these aggregates do not move towards the periphery.
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Figure 2: Colabeling of vasa RNA (red) and microtubules (green) during early (top panels) and late (bottom panels) stages of furrow maturation. An array of microtubules at the furrow, which is affected in nebel mutants (not shown) is requi red for the peripheral translocation of the vasa-RNA-containing aggregate.
Figure 3: Wild type 24-hour embryo (left panel), and embryos mutant for a ventralizing mutation (middle panel) and an anteriorizing mutation (right panel).
